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Gelfand
Lab |
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Regulation
of Organelle Transport |
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In
Drosophila Cell Culture |
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One of the advantages
of using Drosophila cells is the effectiveness of RNA interference (RNAi).
Of all the proteins that we tested so far, the target proteins were consistently
reduced to less than 10% of the control level without affecting other proteins.
By combining the RNAi technology and high-resolution microscopy, individual
components that are involved in transport and transport regulation can be
identified. Furthermore, a novel preparation of Drosophila S2 cells was
developed to generate polarized microtubule arrays in living cells without
the interference of actin filaments, and thus allows us to dissect the microtubule-based
transport machinery directly. |
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S2 cell lines
with organelles (peroxisome, lysosome, mitochondria) labeled with different
fluorescent proteins have been generated in the lab. We are using these
cell lines to study mechanisms of intracellular transport and its regulations. |
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Drosophila
S2 cells expressing GFP-SKL (peroxisome targeting signal) and mCherry-tubulin. |
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| Last
updated on January 8, 2008 |
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©
Gelfand Lab - Northwestern University |
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